Intracytoplasmic sperm injection (ICSI)

Simulation Program in Embryology

Intracytoplasmic sperm injection (ICSI) is a key technique in Assisted Human Reproduction (AHR).

It is a mechanical and delicate process that requires specific skills and competencies. In our country, the way by which embryologists acquire skills and competencies in ICSI is not easily reproducible and depends on the laboratory and the workload there. It involves microinjecting discarded oocytes which do not contain spermatozoids, and sperm immobilisation.

There is no data supporting a minimum number of microinjections needed to obtain the competency, nor a skill-acquisition evaluation system. On the other hand, the learning curve can be long if there aren’t a high number of cycles.

The final evaluation of competencies is performed starting with the microinjection of groups of oocytes from egg donation cycles under the supervision of senior embryologists once it’s been subjectively determined that the technique has been dominated. Simulation training by performing microinjection on mouse gametes makes it possible to acquire skills and competencies in ICSI in a way that can be reproduced and evaluated before moving on to the Assisted Human Reproduction laboratory.

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